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ASCP Histotechnologist International (ASCP-HTLI) Practice Tests & Test Prep by Exam Edge - Topics


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ASCP Histotechnologist International Exam Blueprint

Understanding the exact breakdown of the ASCP Histotechnologist International test will help you know what to expect and how to most effectively prepare. The ASCP Histotechnologist International has multiple-choice questions The exam will be broken down into the sections below:

ASCP Histotechnologist International Exam Blueprint
Domain Name % Number of
Questions
Fixation 15-25% 19
Processing 10-20% 13
Embedding/Microtomy 15-25% 19
Staining 30-40% 38
Laboratory Operations 10-15% 13

ASCP Histotechnologist International - Exam Topics Sample Questions

Neuroglia is composed of Schwann cells found where?

Correct Answer:
peripheral nervous system.
neuroglia, also known as glial cells, plays a crucial role in supporting neurons in the nervous system. unlike neurons, neuroglia do not conduct electrical impulses but perform many essential functions that help maintain the health and structure of the nervous system.

schwann cells, a type of neuroglia, are found specifically in the peripheral nervous system. the peripheral nervous system (pns) comprises nerves and ganglia outside of the brain and spinal cord. it is responsible for connecting the central nervous system (cns) to limbs and organs, essentially serving as a relay between the brain and spinal cord and the rest of the body.

schwann cells are vital for their role in nerve regeneration and for producing the myelin sheath in the peripheral nervous system. the myelin sheath is a fatty layer that surrounds the nerve fibers and is crucial for the proper transmission of electrical signals along the nerve cells. this sheath not only speeds up the electrical impulses but also provides insulation and protection to the nerve fibers.

in contrast to the schwann cells in the pns, the central nervous system contains oligodendrocytes, which also produce a myelin sheath but for neurons within the brain and spinal cord. other types of neuroglia in the cns include astrocytes, which are involved in forming the blood-brain barrier and regulating blood flow, and microglia, which act as the first and main form of active immune defense in the cns.

understanding the distribution and function of different types of neuroglia, including schwann cells, is crucial for medical science, particularly in addressing diseases and injuries of the nervous system. for example, damage to schwann cells in the peripheral nervous system can lead to peripheral neuropathies, whereas issues with oligodendrocytes in the cns can contribute to conditions like multiple sclerosis.

thus, the correct answer to the question, "neuroglia is composed of schwann cells found where?" is the "peripheral nervous system." this highlights the specialized roles that different glial cells play in distinct parts of the human nervous system, emphasizing their critical support functions in both neural health and disease.

When is acetone used for tissue processing?

Correct Answer:
for small, hand processed tissues.
acetone is used in tissue processing specifically for small, hand-processed tissues.

processing tissue is a crucial step in histological studies, allowing for the detailed examination of cell structures under a microscope. once the tissue is fixed to preserve its structure and halt any biological processes, it must be prepared to be embedded into a medium like paraffin wax. this embedding process makes it possible to cut the tissue into very thin slices, or sections, which are essential for microscopic examination.

tissue processing involves several key steps, including dehydration and clearing. dehydration is the process of removing water from the tissue, usually by passing it through increasing concentrations of alcohol. clearing follows dehydration and involves replacing the alcohol with a substance that prepares the tissue for infiltration with paraffin.

acetone is sometimes used as a dehydrating and clearing agent in the processing of small, manually handled tissue samples. its primary advantage is its rapid action, which can significantly speed up the processing time. this can be particularly beneficial in research settings where time is a critical factor. however, the use of acetone also comes with notable drawbacks, mainly its high flammability, which poses a significant fire risk. this risk requires stringent safety measures to be in place when using acetone in a laboratory setting.

due to its rapid action and the safety risks associated, acetone is generally reserved for use with small, hand-processed tissues where its benefits in speeding up the process outweigh its disadvantages. for larger, machine-processed tissues, other chemicals are typically preferred that offer a safer or more suitable alternative for handling larger volumes or automated processing systems.

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