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ASCP Histotechnologist International - Blogs Sample Questions

Which of the following leaves the slides in the solution for a specific time period and then they are placed through a solution that removes some of that stain?

Correct Answer:
regressive stain.


the correct answer to the question is "regressive stain." this type of staining technique involves initially overstaining the tissue sample and then selectively removing the excess stain, allowing for enhanced contrast and detail in the microscopic examination of tissues.

staining is a crucial technique in histology, which is the study of the microscopic structure of tissues. it enhances the contrast in the microscopic images by adding color to different parts of the tissue, which would otherwise be transparent and difficult to distinguish under a microscope. one of the most commonly used staining protocols is hematoxylin and eosin (h&e) staining. hematoxylin, which colors cell nuclei blue, must be oxidized to become a staining agent. eosin, on the other hand, stains the cytoplasmic components, connective tissues, and muscle fibers in varying shades of pink.

in the context of hematoxylin staining, there are two primary methods: progressive and regressive staining. in progressive staining, the tissue sections are stained to the desired intensity directly. this method offers more control over the staining intensity as the stain is built up gradually.

conversely, regressive staining involves initially overstaining the tissue sections, which means that the stain is applied more heavily than needed. the key step in regressive staining is the differentiation or destaining process. after the slides are overstained, they are subjected to a solution that selectively removes some of the stain. this process is controlled carefully to achieve the desired staining intensity and to highlight specific features of the tissue. this method is particularly useful for staining large batches of slides, where uniform staining across multiple samples is crucial.

therefore, the answer "regressive stain" specifically refers to the practice where slides are initially left in a staining solution for a set period to overstain them, followed by a treatment with a differentiating solution that removes the excess stain, thereby achieving the required contrast and detail for microscopic analysis.

Which of the following is used for indicating starch?

Correct Answer:
iodine.


the correct answer to the question "which of the following is used for indicating starch?" is iodine. iodine is a chemical element that, when used as a stain, interacts specifically with starch to produce a color change, making it visible under certain conditions. this characteristic is utilized extensively in both educational settings and various scientific fields to identify and quantify the presence of starch in samples.

staining techniques, in general, are crucial in biological and chemical sciences for highlighting structures in microscopic tissues, cells, or other materials that are otherwise difficult to see. stains bind to specific components within cells or tissues, providing a contrast that is visible under a microscope.

among the options given: - hoechst stains are primarily used for staining dna in live or fixed cells and are known for their blue fluorescence under uv light. - dapi (4',6-diamidino-2-phenylindole) is another nuclear stain that binds to dna, emitting a blue fluorescence when exposed to ultraviolet light. - ethidium bromide is a compound commonly used to visualize nucleic acids in gel electrophoresis experiments. it intercalates into dna and rna, fluorescing under uv light.

however, only iodine is used for indicating the presence of starch. when iodine is added to a sample containing starch, it slips into the helical structure of the amylose, a component of starch, and the complex formed reflects a dark blue or purple color. this reaction is specific to starch, making iodine an excellent indicator for this polysaccharide in various applications including food testing and educational experiments.

Additional Blogs for ASCP - American Society for Clinical Pathology dfgdfgdfg

In your journey to get ASCP Histotechnologist International certified it is important for you to have all information related to your exam. So we have pulled together a list of additional blogs that may be of interest to you because that are all related to the American Society for Clinical Pathology.

Exam Edge Blogs for ASCP - American Society for Clinical Pathology